Description:RecombinantmonoclonalantibodytoV(beta)3TCR.ManufacturedusingAbAb’sRecombinantPlatformwithvariableregions(i.e.specificity)fromthehybridomaJOVI.1.
ThischimerichumanantibodywasmadeusingthevariabledomainsequencesoftheoriginalMouseIgG2aformat,forimprovedcompatibilitywithexistingreagents,assaysandtechniques.
SpeciesandIsotype:HumanIgG1,kappa
CloneNumber:JOVI.1
UniProtAccessionNumberofTargetProtein:PublishedApplication(s):FACS;IHC;IF;IP
PublishedSpeciesReactivity:Human
Immunogen:Thymus,spleenandmesentericlymphnodesisolatedfromamousetransgenicforhumanVb3TcR.
Specificity:ThisantibodybindstohumanV-beta3chainoftheT-cellreceptor.InparticularthisantibodybindstotheTRBC1variantbutnottheTRBC2variant.
ApplicationNotes:ThisantibodycanbeusedtodetecttheTCRbetachaininamajority(50-75%)ofT-cellreceptorsbyflowcytometry,immunofluorescenceandcanimmunoprecipitatetheTCRbetaprotein(variant1).RecentlyithasbeencombinedintoachimericantigenantigenreceptortherapyapproachtoselectivelydepleteasubsetofT-cellsinT-celllymphomasorleukaemias,whilstleavingthenon-reactiveT-cellsubsetintact.
AntibodyFirstPublishedin:Vineyetal.GenerationofmonoclonalantibodiesagainstahumanTcellreceptorbetachainexpressedintransgenicmice.Hybridoma.1992Dec;11(6):701-13PMID:1284120
Noteonpublication:Describesthegenerationandinitialcharacterisationofthereactivityofthisantibodyclone.
ProductForm
Purification:ProteinAaffinitypurified
Suppliedin:PBSonly.
StorageRecommendation:Storeat4°Cforupto3months.Forlongertermstoragealiquotintosmallvolumesandstoreat-20°C
Concentration:Seeviallabel.
Importantnote–Thisproductisforresearchuseonly.Itisnotintendedforuseintherapeuticordiagnosticproceduresforhumansoranimals.