Description:RecombinantmonoclonalantibodytoE-proteinDIII(ABDE).ManufacturedusingAbAb’sRecombinantPlatformwithvariableregions(i.e.specificity)fromthehybridomaZV-2.
SpeciesandIsotype:HumanIgM,kappa
CloneNumber:ZV-2
UniProtAccessionNumberofTargetProtein:
PublishedApplication(s):crystallization;SPR;FC
PublishedSpeciesReactivity:ZikaVirus
Immunogen:Thisantibodywasgeneratedbyinfectingmicewith1000FFUofZIKVMR-766followedbyaboosterat30dpost-infectionofZIKVH/PF/2013,respectivelyandgivenafinalintravenousboostwithliveZIKV(H/PF/2013).
Specificity:ZV-2bindstotheZikavirusenvelopeproteinatdomainIII(DIII,ABDEsheet).Nocross-reactivitywithothertestedflaviviruseshasbeenreported.
ApplicationNotes:TheoriginalmouseIgG2cformathasbeenusedtolabelZIKVinfectedcells(4%PFAfixation,0.1%saponinpermeABIlization).Itwasnotfoundtohaveneutralizingactivity.
AntibodyFirstPublishedin:
Zhaoetal.StructuralBasisofZikaVirus-SpecificAntibodyProtection.Cell.2016Aug11;166(4):1016-27Noteonpublication:Describesthegenerationandcharacterisationofthisantibody.

ProductForm

Purification:AffinityPurifiedusingarecombinantlectincolumn
Suppliedin:PBSwithpreservative(0.02%Proclin300)
StorageRecommendation:Storeat4°Cforupto3months.Forlongertermstoragealiquotintosmallvolumesandstoreat-20°C
Concentration:Seeviallabel.