Description:RecombinantmonoclonalantibodytoEbolaNucleoprotein.ManufacturedusingAbAb’sRecombinantPlatformwithvariableregions(i.e.specificity)fromthephagedisplayantibodyKZ51.
SpeciesandIsotype:MouseIgG1,kappa
CloneNumber:KZ51
UniProtAccessionNumberofTargetProtein:
PublishedApplication(s):ELISA;WB;IP;IF
PublishedSpeciesReactivity:EbolaVirus(Zaire,IvoryCoast,Reston,Sudan)
Immunogen:ThisantioBDywasderivedfromahumansurvivorofthe1995KikwitoutbreakofEbola.
Specificity:ThisantibodybindstothenucleoproteinoffourEbolavirusstrainsandhasaspecificityfoundinconvalescentpatients.Nocross-reactivitywithotherproteins(e.g.GP)wasdetectedbyrADIoimmunoprecipitation.
ApplicationNotes:Thisantibodyisbroadlycross-reactingbetweendifferentEbolastrainsandcompeteswithantibodiesnaturallyoccuringincovalescentpatients.ByIF,thisantibodyhashigherreactivitytowardsfixedvirusthantolivevirus-thoughbothmaybedetected.Ithasalsobeenusedasapositivecontrolinthedevelopmentofanti-Ebolapolyclonalgoatantibodies(Boisenetal.2015).
AntibodyFirstPublishedin:
Maruyamaetal.Ebolaviruscanbeeffectivelyneutralizedbyantibodyproducedinnaturalhumaninfection.JVirol.1999Jul;73(7):6024–6030.PMID:12350354Noteonpublication:Describesthegenerationandfunctionalcharacterizationofthisantibody.

ProductForm

Purification:ProteinAaffinitypurified
Suppliedin:PBSwithpreservative(0.02%Proclin300)
StorageRecommendation:Storeat4°Cforupto3months.Forlongertermstoragealiquotintosmallvolumesandstoreat-20°C
Concentration:Seeviallabel.