UniProt Accession Number of Target Protein: P00533
Alternative Name(s) of Target: EGFR, ErbB-1, HER1; epidermal growth factor receptorImmunogen: The epidermal growth factor receptoris the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands.
Specificity: Recognises human EGFR.
Antibody first published in:Prewett M et al.The biologic effects of C225, a chimeric monoclonal antibody to the EGFR, on human prostate carcinoma.J Immunother Emphasis Tumor ImmunolPMID:9041461Note on publication:Describes effect of antibody on human prostate cancer.
Immunohistochemical staining of rat stomach tissue using anti-EGFR antibody (Ab00279) C225 (Cetuximab) Anti-EGFR staining of formaldehyde fixed paraffin embedded rat stomach tissue, at 40x magnification. The human IgG1-chimeric version of C225 (Ab00279-10.0) was used to stain samples at a concentration of 5 µg/ml.
Binding curve of anti-EGFR antibody to EGFR-Fc fusion protein ELISAPlate coated with human EGFR-Fc (Pr00117-10.9) at a concentration of 2.0 mg/ml. A 2-fold serial dilution from 100 to 0.1 ng/ml was performed using HRP labelled cetuximab human IgG1 (Ab00279-10.0).
Absolute Antibody于2012年在英国剑桥成立,是由英国牛津大学Hutchings博士和Barclay教授创办的第二代抗体公司,可以在两周内提供100mg级的工程化抗体,并且不含动物成分,批间差极小,消除因补体和Fc受体造成的背景,使结果更干净。该公司的成立顺应了Nature杂志为首的科学界提倡改变目前抗体供应混乱状况的时代潮流。目前提供国内的产品主要包括:
l 科研用生物仿制药抗体
l 嵌合单克隆抗体
l 用于体内实验的重组抗体
l 表位标签
l 重组非IgG同型对照
l 重组蛋白(Fc融合蛋白)