Immunofluorescence staining of fixed mouse splenocytes with anti-B7-H3 (CD276) antibody MJ18 (Ab00884) Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix™ coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of MJ18 (Ab00884-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing weak membrane staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00884-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (Ab102-23.0) followed by Alexa Fluor® 488 secondary antibody

Binding curve of anti-B7-H3 antibody MJ18 (Ab00884-23.0) to mouse B7-H3-Fc fusion protein ELISA Plate coated with mouse B7-H3-Fc fusion protein (Pr00158-1.9) at a concentration of 5 µg/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using Ab00884-23.0. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used.