UniProt Accession Number of Target Protein: P41274
Alternative Name(s) of Target: CD137L; TNFRSF9L; CD134 ligand; 4-1BB Ligand; TNFRSF9; TNFR superfamily 9; tumor necrosis factor receptor superfamily member 9Immunogen: Murine B-cell lymphoma 2PK-3 cells injected into SD rats.
Specificity: This antibody binds to murine 4-1BBL and competes with binding of 4-1BB.
Application Notes: This antibody has been used to detect 4-1BBL by flow cytometry. It is able to block 4-1BBL interacting with 4-1BB and prevent signalling.
Antibody first published in:Futagawa et al.Expression and function of 4-1BB and 4-1BB ligand on murine dendritic cells.Int Immunol. 2002 Mar;14(3):275-86PMID:11867564Note on publication:Describes the generation and initialcharacterisation of this antibody.
ELISA of anti-4-1BBL antibody on 4-1BBL-Fc fusion protein. Binding curves of the rabbit IgG chimeric version of the anti-4-1BBL antibody TKS-1 (Ab00881-23.0; red line) and isotype control (Ab00102-23.0, anti-Fluorescein; blue line) to an ELISA plate coated with mouse 4-1BBL Fc-fusion protein (Pr00129-1.28) at a concentration of 5 µg/ml. A 3-fold serial dilution from 10,000 to 0.17 ng/ml was performed using Ab00881-23.0. For signal detection, a 1:4000 dilution of HRP-fused anti-rabit IgG antibody was used.
Absolute Antibody于2012年在英国剑桥成立,是由英国牛津大学Hutchings博士和Barclay教授创办的第二代抗体公司,可以在两周内提供100mg级的工程化抗体,并且不含动物成分,批间差极小,消除因补体和Fc受体造成的背景,使结果更干净。该公司的成立顺应了Nature杂志为首的科学界提倡改变目前抗体供应混乱状况的时代潮流。目前提供国内的产品主要包括:
l 科研用生物仿制药抗体
l 嵌合单克隆抗体
l 用于体内实验的重组抗体
l 表位标签
l 重组非IgG同型对照
l 重组蛋白(Fc融合蛋白)