Description:RecombinantmonoclonalantibodytoLymphotoxinalpha.ManufacturedusingAbAb’sRecombinantPlatformwithvariableregions(i.e.specificity)fromthehybridomaMLTA3698A(Pateclizumab).
SpeciesandIsotype:HumanIgG1,kappa
CloneNumber:MLTA3698A(Pateclizumab)
UniProtAccessionNumberofTargetProtein:
PublishedApplication(s):FC;SPR;block;deplete
PublishedSpeciesReactivity:Human
Immunogen:Thishumanizedantibodywasderivedfromamouseanti-humanLT-ahybridomamAb.
Specificity:Thisantibodyisspecificforlymphotoxin-alpha,andisabletobindboththesolubleLTα3homotrimericformandthecell-surfaceLTα1β2heterotrimer,whichistransientlyexpressedonactivatedBandTcells(Th1,Th17).
ApplicationNotes:ThisantibodyhasbeenshowntoabletobothblockLTα-mediatedsignalingandselectivelydepleteLTα-expressingactivatedTandBcells,whichhastherapeuticapplicationsinabrogatingproinflammatoryresponsesinrheumatoidarthritis(RA)pathology(Kennedy,2014).PreliminaryclinicaltrialsdemonstratedthatadmiNISTrationofthisantibodydecreasedserumlevelsofCXCL13,abioMarkerofRAdiseaseactivity(Emu,2012).InaphaseIItrial,thisantibodyhadatrendforclinicaleffectsonthesymptomsofRAafter3monthsoftreatment,butthedifferencesfromplaceboresponseswerenotstatisticallysignificant(Kennedy,2014).InahumanperipheralbloodmononuclearcelltransplantedSCIDmousemodelofgraft-versus-hostdisease,thisantibodyspecificallydepletedactivatedLTα-expressinghumandonorTandBcells,prolongingsurvivalofthemice(Chiang,2012).Finally,thisantibodyhasbeenusedtoinFCandsurfaceplasmonresonanceanalysistocharacteriseitsinteractionwiththeLTαligand.
AntibodyFirstPublishedin:
Chiangetal.InVivoDepletionofLymphotoxin-AlphaExpressingLymphocytesInhibitsXenogeneicGraft-versus-Host-DiseasePLoSONE,2012,Vol.7(3),p.e33106PMID:22427961Noteonpublication:DescribesthegenerationofthisantibodyanditsuseinprolongingsurvivalinamousemodelofGVHD.

ProductForm

Purification:ProteinAaffinitypurified
StorageRecommendation:Storeat4°Cforupto3months.Forlongertermstoragealiquotintosmallvolumesandstoreat-20°C
Concentration:Seeviallabel.