Description:RecombinantmonoclonalantibodytoDR5.ManufacturedusingAbAb’sRecombinantPlatformwithvariableregions(i.e.specificity)fromthetherapeuticantibodyPRO95780(Drozitumab).
SpeciesandIsotype:RabbitIgG,lamBDa
CloneNumber:PRO95780(Drozitumab)
UniProtAccessionNumberofTargetProtein:O14763
PublishedApplication(s):functional/therapeutic;agoNIST;IP;immunoblot;ELISA;IF
PublishedSpeciesReactivity:Human
Immunogen:ThevariabledomainsofthisantibodywerederivedasscFvfromahumanantibodyphagedisplaylibrary,withbiotinylatedDR5-FcusedtocaptureDR5-specificphage.ThescFvobtainedweresubsequentlyreformattedintofull-lengthantibodies.
Specificity:Thisantibodyisspecificforhumandeathreceptor5,aproapoptoticreceptorwidelyexpressedinadultandfetaltissues,withnotablyhighexpressionontumourcells.ActivationofDR5triggersapoptosisinvarioustypesofcancercells,withoutaffectingmostnormalcelltypes.
ApplicationNotes:ThisantibodybindsDR5,triggeringDR5clusteringandDISCactivationinarangeofcancercells,includingcolorectalcancerandNSCLCcells(Adams,2009).Conversely,thisantibodyshowednoeffectorcaspaseactivationorcelldeathinductioninnormalhumanhepatocytes.Invivo,thisantibodyhasbeenshowntoinhibittumorxenograftgrowthinavarietyofanimalmodels(Varfolomeev,2009).ThisantibodywasusedinIP,immunoblot,ELISAandIFanalysistopreciselycharacteriseitsinteractionwiththeDR5ligand(Adams,2009).
AntibodyFirstPublishedin:
Adamsetal.StructuralandfunctionalanalysisoftheinteractionbetweentheagonisticmonoclonalantibodyApomabandtheproapoptoticreceptorDR5.CellDeathDiffer.2008Apr;15(4):751-61PMID:18219321Noteonpublication:Describesthegenerationofthisantibodyfromaphagedisplaylibrary,andsubsequentcharacterisationofitsbindingspecificity.

ProductForm

Purification:ProteinAaffinitypurified
StorageRecommendation:Storeat4°Cforupto3months.Forlongertermstoragealiquotintosmallvolumesandstoreat-20°C
Concentration:Seeviallabel.