Description:RecombinantmonoclonalantibodytoLy6G.ManufacturedusingAbAb’sRecombinantPlatformwithvariableregions(i.e.specificity)fromthehybridoma1A8.
SpeciesandIsotype:RatIgG2a,kappa
CloneNumber:1A8
UniProtAccessionNumberofTargetProtein:P35461
AlternativeName(s)ofTarget:Ly-6G;GR-1;GR1;Lymphocyteantigen6G
PublishedApplication(s):FC;depletion
PublishedSpeciesReactivity:Mouse
Immunogen:LEW/Nratsimmunizedwith1.5x10E8Ly-6G-transfectedEL4Jcells
Specificity:RecognisesthemurineGPI-anchoredproteinLy6G,aMarkerofgranulocytedifferentiation,maturationand,togetherwithLy6C,acomponentoftheGranulocyteReceptor-1Antigen(Gr-1).Unlikeanothercommonlyusedclone,RB6-8C5,1A8doesnotbindLy6C.
ApplicationNotes:Neutrophilsarecrucialcomponentsofourimmunesystem.Ly6Gishighlyexpressedonneutrophils,whichallowsthe1A8clonetobeusednotonlyindetectionofneutrophils,butalsoinselectivedepletioninvivo.Itmayalsobeusedasanegativeselectionmarkerforbone-marrowderivedmacrophages,whichshouldbeLy6G(-)andF4/80(+)(seeourF4/80rangeofantibodies).
AntibodyFirstPublishedin:
FlemingTJ,FlemingML,MalekTR.SelectiveexpressionofLy-6Gonmyeloidlineagecellsinmousebonemarrow.RB6-8C5mAbtogranulocyte-differentiationantigen(Gr-1)detectsmembersoftheLy-6family.JournalofImmunologyPMID:8360469Noteonpublication:Describesproductionof1A8andidentificationofLy6Gasitstarget.

ProductForm

Purification:ProteinAaffinitypurified
StorageRecommendation:Storeat4°Cforupto3months.Forlongertermstoragealiquotintosmallvolumesandstoreat-20°C
Concentration:Seeviallabel.