Description:RecombinantmonoclonalantibodytoHIV-1/HIV-2protease.ManufacturedusingAbAb’sRecombinantPlatformwithvariableregions(i.e.specificity)fromthehybridoma1696.ThisisaFabfragmentwithahis-tag.
SpeciesandIsotype:MouseFabfragment,His-Tagged,kappa
CloneNumber:1696
UniProtAccessionNumberofTargetProtein:
PublishedApplication(s):Neutralize;ELISA;WB
PublishedSpeciesReactivity:HIV-1;HIV-2
Immunogen:1696waspreparedbygeneratingahybridomacellline,fusingmyelomacellswithsplenocytesderivedfromBALB/cmiceimmunizedwithHIV-1PRinthepresenceofFreund"sadjuvant.
Specificity:1696bindstoHIV-PRofbothHIV-1andHIV-2(IgG1696:HIV-1PRpH5.7~350uM;pH7.4~100nM-HIV-2PRpH5.7nomeasurableinteraction;pH7.4~30nM--Fab1696:HIV-1PRpH5.7~410nM;pH7.4~130nM-HIV-2PRpH5.7nomeasureableinteraction;pH7.4~40nM--determinedbyELISA).TheantibodybindstotheN-terminalregionoftheenzyme,andbindingisinhibitedbythepeptidefragmentPQITLWQwhichrepresentsresidues1-7.Westernblotbindingassaysshowthat1696recognizesthematureandprocessedformoftheenzyme,butnottheprecursorwhichpossesses20residuesupstreamfromthecleavagesite.TheN-terminushasawellconservedstructurebetweenHIV-1PRandHIV-2PR.HIV-PRisahomodimericapartateproteasethatspecificallycleavestheviralGagandGag/Polpolyproteinprecursor.Acorrelationhasbeenobservedbetweentheactivityoftheproteaseandthedegreeofinfectivity,andsothisproteinisamajorantiviraltarget.
ApplicationNotes:1696mAbcaninhibitthecatalyticactivityofbothHIV-1andHIV-2HIV-PR(1696IgGHIV-1PRIC50~0.6nM;HIV-2PRIC50~1.5nM--1696FabHIV-1PRIC50~1.1nM;HIV-2PRIC50~2.6nM).Inhibitionby1696ismediatedbythedestABIlizationoftheoftheactiveformoftheactiveHIV-PRhomodimerthroughbindingtotheN-terminus,whichconstitutesalargepercentageoftheinterfacebetweenthemonomers.TheFabfragmentwasgeneratedbyincubationoftheantibodywithpepsin.Bindingconstantsof1696canbedeterminedbyELISA.1696showsreactivitytothematureprocessedHIV-PRandnottotheprecursorbyWB.
AntibodyFirstPublishedin:
Lescaretal.InhibitionoftheHIV-1andHIV-2proteasesbyamonoclonalantibodyProteinSci.1999Dec;8(12):2686–2696.Noteonpublication:DescribesthegenerationofthemAb1696,itsfunctionalcharacterizationandthedeterminationofthecrystalstructureoftheFabfragment.

ProductForm

Purification:PurifiedbyImmobilizedMetalAffinityChromatography
Suppliedin:PBSwithpreservative(0.02%Proclin300)
StorageRecommendation:Storeat4°Cforupto3months.Forlongertermstoragealiquotintosmallvolumesandstoreat-20°C
Concentration:Seeviallabel.