Description:RecombinantmonoclonalantibodytoGlycoproteinH.ManufacturedusingAbAb’sRecombinantPlatformwithvariableregions(i.e.specificity)fromthehyBDridomaA13-d6.3.ThisantibodywascreatedusingourproprietaryFcSilent™engineeredFcdomaincontainingkeypointmutationsthatabrogatebindingtoFcgammareceptors.
SpeciesandIsotype:HumanIgG1,FcSilent™,kappa
CloneNumber:A13-d6.3
UniProtAccessionNumberofTargetProtein:
PublishedApplication(s):neutralize;crystalize;immunostaining
PublishedSpeciesReactivity:pseudorABIesvirus(PrV)
Immunogen:d6.3wasgeneratedbyimmunizingmicewithPrV.
Specificity:d6.3FabfragmentbindstothecorefragmentofthePrVglycoproteinH(gH)ectodomain(aa107–639)whichisthemembrane-proximalendoftheproteininthe?gL-PassPrVvariantofPrV.Theantibodycontactsresidues332-373(peripheralhelicesα6andα7ofdomainIIIofgHcontributemostoftheresiduesthatformtheepitoperecognizedbyd6.3).PrVisaporcineherpesvirusbelongingtothealpha-HerpesvirinaesubfamilyandisknowntocauseAukeszky"sdiseaseinpigs.gHisatypeImembraneporteinandcaninteractnon-covalentlywithgLviathelargegHectodomain.
ApplicationNotes:d6.3 neutralizesthe?gL-PassPrVvariantofPrVandcanstain?gL-PassPrVvariantbutnotthewild-typePrVbyimmunostaining.Theepitopeofd6.3isnotblockedbytheformationofthegH/gLheterodimer.Thed6.3Fabfragmnet(obtainedthroughpapaindigestion)canbecomplexedwiththecorefragmentofthePrVglycoproteinH(gH)ectodomain(aa107–639)forstructuralstudiesbycrystallography.
AntibodyFirstPublishedin:
Backovicetal.StructureofacorefragmentofglycoproteinHfrompseudorabiesvirusincomplexwithantibody.ProcNatlAcadSciUSA. 2010Dec28;107(52):22635-40PMID:21149698Noteonpublication:DescribesthedeterminationofthecrystalstructureofPrVgHcomplexedwithd6.3andthemodeofantibodybinding.ThesolvedstructureisalsousedtocomparePrVgHwiththatofEpstein-Barrvirus(EBV).

ProductForm

Purification:ProteinAaffinitypurified
Suppliedin:PBSwithpreservative(0.02%Proclin300)
StorageRecommendation:Storeat4°Cforupto3months.Forlongertermstoragealiquotintosmallvolumesandstoreat-20°C
Concentration:Seeviallabel.