Description:RecombinantmonoclonalantibodytoEnvelopeprotein.ManufacturedusingAbAb’sRecombinantPlatformwithvariableregions(i.e.specificity)fromtheEBVtransformedhumanBcellcloneZKA78.
SpeciesandIsotype:HumanIgM,kappa
CloneNumber:ZKA78
UniProtAccessionNumberofTargetProtein:
PublishedApplication(s):NTRL;enhance;FC;ELISA
PublishedSpeciesReactivity:ZikaVirus;DengueVirus
Immunogen:mAbZKA78wasselectedfromEBV-immortalizedmemoryBcellsderivedfromZIKV-infected,DENV-naïvehumandonorsbasedonitsABIlitytobindZikavirus(ZIKV)EnvelopeproteinandtoneutralizeZIKVinfection.
Specificity:ZKA78isspecificforEDI/IIdomainoftheZIKVenvelope(E)protein,andcross-reactswiththeEproteinfromDenguevirus(DENV),whichishighlyhomologous(alsocontainshighsequencesimilaritywithotherflavivirusessuchasyellowfeverandwestnile).ZKA78showedsignificantbinding(determinedbyELISA)totheEproteinsofZIKV,DENV1-4andthevirus-likeparticles(VLPs)ofDENV1-4.FlavivirusEproteinsmediatefusion,withEDIbeinginvolvedinconformationalchangesrequiredforviralentryandEDIIcontainingafusionloop.ZIKVisaflavivirusthatcanbetransmittedsexually,bytheAedesmosquitovector,orverticallytoadevelopingfetus.MostcasesofZIKVinfectionareasymptomaticormanifestinmildsymptoms,butinsomecasesZIKVinfectioncanresultinGuillain-BarréSyndromeorcongenitalbirthdefectsindevelopingfetuses.
ApplicationNotes:ZKA78isabletopartiallyneutralizeZIKVinfectivity(IC50~2863ng/ml),andiscapableofenhancingZIKV/infectionofK526cellsviatheantibody-dependentenhancement(ADE)effect.Thisantibodycanalsoneutralize(IC50~266ng/ml)andenhance(byADE)DENVinfection.NeutralizationofZIKVinfectioncanbemeasuredusingamicro-neutralizationFC-basedassay,andbinding/specificitytoEproteincanbestudiedbyELISA.DENV2-infectedAG129miceadmiNISTeredwithZKA78resultsindeathafter5days.
AntibodyFirstPublishedin:
Stettleretal.Specificity,cross-reactivityandfunctionofantibodieselicitedbyZikavirusinfection.Science. 2016Jul14.pii:aaf8505. PMID:27417494Noteonpublication:DescribestheisolationofapanelofantibodiesderivedfromZIKV-infected,DENV-naïveandZIKV-infected,DENV-immunehumandonors.TheyweretestedfortheirabilitytobindtoZikaEproteinorNS1aswellastheircross-reactivitywiththehomologsinDENV.ThecapabilitytoneutralizeZIKVinfectionwasstudiedaswellastheantibody-dependentenhancementeffectoninfection.

ProductForm

Purification:AffinityPurifiedusingarecombinantlectincolumn
Suppliedin:PBSwithpreservative(0.02%Proclin300)
StorageRecommendation:Storeat4°Cforupto3months.Forlongertermstoragealiquotintosmallvolumesandstoreat-20°C
Concentration:Seeviallabel.