Description:RecombinantmonoclonalantibodytoEnvelopeprotein(soluble).ManufacturedusingAbAb’sRecombinantPlatformwithvariableregions(i.e.specificity)fromtheB-cellcloneZ3L1.
SpeciesandIsotype:HumanIgG1,lamBDa
CloneNumber:Z3L1
UniProtAccessionNumberofTargetProtein:
PublishedApplication(s):NTRL;crystallisation;ELISA;FC;SPR
PublishedSpeciesReactivity:Zikavirus
Immunogen:Anti-ZIKVmAbswereobtainedfromtheseraandperipheralbloodmononuclearcellsofaZIKV-infectedpatient.
Specificity:Z3L1bindstotheZIKVsolubleenvelope(sE)protein,shownusingSPR,kineticcompetitionassaysandcrystallography(Wangetal,2016).ThismAbishighlyspecificforZIKV,andwasshowntoexhibitnoneutralisationactivityagainstDENV-1toDENV-4virions,whicharehighlyrelatedtoZIKV.ItwasshowncrystallographicallythattheZ3L1FabregionbindstodomainIanddomainIIofsE.ZIKVhasrecentlybecomeamajorglobalhealthconcern,followinga2015outbreak.Viralinfectionisassociatedwithneurologicalcomplicationsandmicroencephaly.
ApplicationNotes:Z3L1wasconfirmedtobindZIKVsEproteinbyELISA,withanaffinityof5.39uMshownbySPR(Wangetal,2016).UsingaFACS-basedassay,itwasshownthatZ3L1wassufficientforcompleteneutralisationofviralactivityagainstinfectedC6/36andVerocells.Invivo,ithasbeenshownthatZ3L1protectedIFNreceptorKOmicefromZIKVinfection.
AntibodyFirstPublishedin:
Wangetal.MoleculardeterminantsofhumanneutralizingantibodiesisolatedfromapatientinfectedwithZikavirus.SciTranslMed.2016Dec14;8(369):369ra179.Noteonpublication:Describestheidentificationofseveralanti-ZIKVmAbsfromthebloodofapatientwithZIKVinfection.ThemAbsidentifiedwerecharacterisedforantigenspecificityusingbiochemicalandbiophysicalmethods,andafewofthemAbsfoundwerecrystallisedwiththeZIKVsEprotein.

ProductForm

Purification:ProteinAaffinitypurified
Suppliedin:PBSwithpreservative(0.02%Proclin300)
StorageRecommendation:Storeat4°Cforupto3months.Forlongertermstoragealiquotintosmallvolumesandstoreat-20°C
Concentration:Seeviallabel.