Description:RecombinantmonoclonalantibodytoEnvelopeprotein(soluble).ManufacturedusingAbAb’sRecombinantPlatformwithvariableregions(i.e.specificity)fromtheB-cellcloneZ20.
SpeciesandIsotype:HumanIgG1,kappa
CloneNumber:Z20
UniProtAccessionNumberofTargetProtein:
PublishedApplication(s):NTRL;crystallisation;ELISA;FC;SPR
PublishedSpeciesReactivity:Zikavirus
Immunogen:Anti-ZIKVmAbswereobtainedfromtheseraandperipheralbloodmononuclearcellsofaZIKV-infectedpatient.
Specificity:Z20bindstotheZIKVsolubleenvelope(sE)protein,shownusingSPR,kineticcompetitionassaysandcrystallography(Wangetal,2016).ThismAbishighlyspecificforZIKV,butexhibitedactivityagainstDENV-1toDENV-4virionsatveryhighconcentrations.Showncrystallographically,Z20bindsalmostexclusivelytodomainIIofsE.ZIKVhasrecentlybecomeamajorglobalhealthconcern,followinga2015outbreak.Viralinfectionisassociatedwithneurologicalcomplicationsandmicroencephaly.
ApplicationNotes:Z20wasconfirmedtobindZIKVsEproteinbyELISA,withanaffinityof0.16uMshownbySPR(Wangetal,2016).UsingaFACS-basedassay,itwasshownthatZ20potentlyneutralisedviralactivityagainstinfectedC6/36andVerocells.Invivo,Z20wasabletoprotect80%ofIFNreceptorKOmicefromZIKVinfection.
AntibodyFirstPublishedin:
Wangetal.MoleculardeterminantsofhumanneutralizingantibodiesisolatedfromapatientinfectedwithZikavirus.SciTranslMed.2016Dec14;8(369):369ra179.Noteonpublication:Describestheidentificationofseveralanti-ZIKVmAbsfromthebloodofapatientwithZIKVinfection.ThemAbsidentifiedwerecharacterisedforantigenspecificityusingbiochemicalandbiophysicalmethods,andafewofthemAbsfoundwerecrystallisedwiththeZIKVsEprotein.

ProductForm

Purification:ProteinAaffinitypurified
Suppliedin:PBSwithpreservative(0.02%Proclin300)
StorageRecommendation:Storeat4°Cforupto3months.Forlongertermstoragealiquotintosmallvolumesandstoreat-20°C
Concentration:Seeviallabel.