Description:RecombinantmonoclonalantibodytoE-proteinDIII(LR).ManufacturedusingAbAb’sRecombinantPlatformwithvariableregions(i.e.specificity)fromthehybridomaZV-67.ThisantibodywascreatedusingourproprietaryFcSilent™engineeredFcdomaincontainingkeypointmutationsthatabrogatebindingtoFcgammareceptors.
SpeciesandIsotype:HumanIgG1,FcSilent™,kappa
CloneNumber:ZV-67
UniProtAccessionNumberofTargetProtein:
PublishedApplication(s):NTRL;crystallization;SPR;FC
PublishedSpeciesReactivity:ZikaVirus
Immunogen:Thisantibodywasgeneratedbyinfectingmicewith1000FFUofZIKVMR-766followedbyaboosterat30dpost-infectionofZIKVH/PF/2013,respectivelyandgivenafinalintravenousboostwithliveZIKVDIII(aminoacids299to407oftheZIKVEprotein))
Specificity:ZV-67bindstotheZikavirusenvelopeproteinatdomainIII(DIII,LR).Nocross-reactivitywithothertestedflaviviruseshasbeenreported.
ApplicationNotes:TheoriginalmouseIgG2cformathasbeenshowntohaveneutralizingactivityandhasbeenusedtolabelZIKVinfectedcells(4%PFAfixation,0.1%saponinpermeABIlization).
AntibodyFirstPublishedin:
Zhaoetal.StructuralBasisofZikaVirus-SpecificAntibodyProtection.Cell.2016Aug11;166(4):1016-27Noteonpublication:Describesthegenerationandcharacterisationofthisantibody.

ProductForm

Purification:ProteinAaffinitypurified
Suppliedin:PBSwithpreservative(0.02%Proclin300)
StorageRecommendation:Storeat4°Cforupto3months.Forlongertermstoragealiquotintosmallvolumesandstoreat-20°C
Concentration:Seeviallabel.