Description: Recombinant monoclonal antibody to E-protein DIII (C-C'). Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma ZV-64.
Species and Isotype: Mouse IgG2a, kappa
Clone Number: ZV-64
UniProt Accession Number of Target Protein:
Published Application(s): NTRL; crystallization; SPR; FC
Published Species Reactivity: Zika Virus
Immunogen: This antibody was generated by infecting mice with 1000 FFU of ZIKV MR-766 followed by a booster at 30d post-infection of ZIKV H/PF/2013, respectively and given a final intravenous boost with live ZIKV DIII (amino acids 299 to 407 of the ZIKV E protein))
Specificity: ZV-64 binds to the Zika virus envelope protein at domain III (DIII, C-C’ loop). No cross-reactivity with other tested flaviviruses has been reported.
Application Notes: The original mouse IgG2c format has been shown to have neutralizing activity and has been used to label ZIKV infected cells (4% PFA fixation, 0.1% saponin permeabilization).
Antibody First Published in:
Zhao et al. Structural Basis of Zika Virus-Specific Antibody Protection. Cell. 2016 Aug 11;166(4):1016-27 Note on publication: Describes the generation and characterisation of this antibody.

Product Form

Purification: Protein A affinity purified
Supplied in: PBS with preservative (0.02% Proclin 300)
Storage Recommendation: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Concentration: See vial label.