Description:RecombinantmonoclonalantibodytoCD5.ManufacturedusingAbAb’sRecombinantPlatformwithvariableregions(i.e.specificity)fromthehybridomaOX-19.
ThischimericratantibodywasmadeusingthevariabledomainsequencesoftheoriginalMouseIgG1format,forimprovedcompatibilitywithexistingreagents,assaysandtechniques.
SpeciesandIsotype:RatIgG2b,kappa
CloneNumber:OX-19
UniProtAccessionNumberofTargetProtein:P51882
PublishedApplication(s):WB;IHC;inhibit;FC
PublishedSpeciesReactivity:Rat
Immunogen:Ratimmunizedwiththymocyteglycoproteins.
Specificity:ThisantibodybindstoCD5glycoproteinprimarilyexpressedonthymocytesandmatureperipheralTlymphocytes.CD5isamemberofthescavengerreceptorsuperfamilyandprimarilyexpressedonthymocytesandmatureperipheralTlymphocytes,butnotonNKcells,intestinalintraepithelialgamma/deltaTcells.
ApplicationNotes:Thisantibodycanbeusedfordenaturingandnon-denaturingWBanddevelopedusingperoxidase-conjugatedrabbitanti-mouse.ItcanalsobeusedforIHCofcryostatsectionsfromadultratliver.Stainingisalsoobservedonthebrushborderofthesmallintestineandcolonicepithelium,TcellareasoflymphoidcellsbutnotBcellfollicles
AntibodyFirstPublishedin:McCaughanetal.IdentificationofthebilecanalicularcellsurfacemoleculeGP110astheectopeptidasedipeptidylpeptidaseIV:ananalysisbytissuedistribution,purificationandN-terminalaminoacidsequence.Hepatology11:534-5441990
Noteonpublication:DescribesthetissuedistributionofCD26bystainingusingtheantibodyOX-61.OX-61alsowasshowntodepleteCD26activity.
ProductForm
Purification:ProteinAaffinitypurified
Suppliedin:PBSwithpreservative(0.02%Proclin300)
StorageRecommendation:Storeat4°Cforupto3months.Forlongertermstoragealiquotintosmallvolumesandstoreat-20°C
Concentration:Seeviallabel.
Importantnote–Thisproductisforresearchuseonly.Itisnotintendedforuseintherapeuticordiagnosticproceduresforhumansoranimals.