Description:RecombinantmonoclonalantibodytoBeta-galactosidase.ManufacturedusingAbAb’sRecombinantPlatformwithvariableregions(i.e.specificity)fromthescFv13R4.ThisantibodywascreatedusingourproprietaryFcSilent™engineeredFcdomaincontainingkeypointmutationsthatabrogatebindingtoFcgammareceptors.
Thisisachimericantibodycreatedaspartofapanelofferingantibodiesofthesamespecificityindifferentformats(species,isotype,subtypeandmodifiedversions)foruseasisotypecontrols.
SpeciesandIsotype:RatIgG1,FcSilent™,lam
BDa
CloneNumber:13R4
UniProtAccessionNumberofTargetProtein:P16278
AlternativeName(s)ofTarget:beta-gal;Acidbetagalactosidase;Betagalactosidase1;β-galactosidase;β-gal;EBP;ELNR1;Galactosidasebeta1;GLB1;MPS4B
PublishedApplication(s):WB;FC;negativecontrol
PublishedSpeciesReactivity:E.coli
Immunogen:Beta-galactosidase.
Specificity:13R4recognizeswild-typeE.colibeta-galactosidase.
AntibodyFirstPublishedin:MartineauP,JonesP,WinterG.Expressionofanantibodyfragmentathighlevelsinthebacterialcytoplasm.JMolBiol.1998Jul3;280(1):117-27.PMID:9653035
Noteonpublication:DescribestheparentscFv13antibodyimprovedbyfourroundsofrandommutagenesisle
ADIngtooptimisedscFv13R4;scFv13R4wasselectedforitscapacitytofoldcorrectlyinareducingenvironmentsuchasthecytoplasm.
ProductForm
Purification:ProteinAaffinitypurified
Suppliedin:PBSwithpreservative(0.02%Proclin300)
StorageRecommendation:Storeat4°Cforupto3months.Forlongertermstoragealiquotintosmallvolumesandstoreat-20°C
Concentration:Seeviallabel.
Importantnote–Thisproductisforresearchuseonly.Itisnotintendedforuseintherapeuticordiagnosticproceduresforhumansoranimals.