UniProt Accession Number of Target Protein: P08637; Q9ULV2
Alternative Name(s) of Target: CD16; Low affinity immunoglobulin gamma Fc region receptor III-A; CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc receptor III-2; FCGR3A; CD16A; FCG3; FCGR3; IGFR3; Fc-gamma receptor IIIb; YFC120; YTH-120Immunogen: Human neutrophils.
Specificity: Recognises human Fc gamma RIII, a glycosyl moiety or a determinant of which conformation is dependent on the presence of oligosaccharides (PMID: 1706970 Fleit HB).
Antibody first published in:PMID:0
Flow-cytometry using anti-CD16 antibody YFC 120.5 (Ab00191) Human leukocytes were stained with an isotype control (Ab00102-23.0, panel A) or the rabbit-chimeric version of YFC 120.5 (Ab00191-23.0, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.
Absolute Antibody于2012年在英国剑桥成立,是由英国牛津大学Hutchings博士和Barclay教授创办的第二代抗体公司,可以在两周内提供100mg级的工程化抗体,并且不含动物成分,批间差极小,消除因补体和Fc受体造成的背景,使结果更干净。该公司的成立顺应了Nature杂志为首的科学界提倡改变目前抗体供应混乱状况的时代潮流。目前提供国内的产品主要包括:
l 科研用生物仿制药抗体
l 嵌合单克隆抗体
l 用于体内实验的重组抗体
l 表位标签
l 重组非IgG同型对照
l 重组蛋白(Fc融合蛋白)