UniProtAccessionNumberofTargetProtein:P67875

AlternativeName(s)ofTarget:Aspf1;Aspf1;ribotoxinAspf1;ribonucleasemitogillin;allergenAspfI;AspfI;IgE-bindingribotoxin;majorallergenAspf1;allergen:Aspf1Immunogen:4F8antibodywasraisedusinganoveltechnique:mononuclearcellsweretakenfromthehumansubjectswithallergicbronchopulmonaryaspergillosisandthenscreenedforIgEsecretion.Subsequently,theywereusedtogeneratehybridomasofwhichsomeweresecretinganti-Aspf1IgE

Specificity:ThisantibodyrecognisesAspf1.

ApplicationNotes:Thisantibody"stargetprotein(Aspf1)wasconfirmedthroughIPandmassspectrometryanalysis(Wurthetal.,2018).WBandELISA:theantibodywassuccessfullindetectingAspf1antigeninacommercialAspergillusextractALKBinELISAandwesternblot(Wurthetal.,2018).

Antibodyfirstpublishedin:Wurthetal.HumanIgEmAbsdefinevariABIlityincommercialAspergillusextractallergencompositionJCIInsight.2018Oct18;3(20).pii:123387.doi:10.1172/jci.insight.123387.PMID:30333320Noteonpublication:ThepaperdescribesamethodofgeneratingAspergillus-reactiveIgE–secretinghumanhybridomas(amongthemthesesecretingthisantibody).Subsequently,theantibodyischaracterizedanditsspecificityisdetermined.Finally,thisantibodyisusedtodeterminapotentialpresenceofitsantigeninthecommercialy-availableAspergillussamplesusedindiagnosisandtreatmentofAspergillus-relatedallergies.