UniProtAccessionNumberofTargetProtein:Q6PSU2

AlternativeName(s)ofTarget:Arah2;Arachishypogaea;peanut;Arah3;Conglutin;2Salbumin;Conglutin-7;2Sprotein1;SeedstorageproteinSSP1;SeedstorageproteinSSP2Immunogen:ThisantibodywasacquiredbyisolatingIgEBcellsfromperipheralbloodofhumanindividualsallergictopeanutsandsubsequentcloningandexpressingtheantibody(Crooteetal.,2018).

Specificity:ThisantibodyrecognisespredominantlyArah2,butitalsocross-reactswithArah3andweaklywithArah1.Arah2isoneofthemajorpeanutallergens.

ApplicationNotes:ELISAtestsconfirmedthatthisantibodystronglybindsArah2andtoalesserdegreeothers(mostlyArah3)(Crooteetal.,2018).BiolayerinterferometryexperimentsrevealedthatPA12P3D08antibodyexhibitsveryhighaffinitytopeanutallergens(Crooteetal.,2018).Consequently,thisantibodyanditsengineeredversions(suchasanIgGversionwiththesamevariabledomainsastheoriginalIgE)mightbeusedinthedevelopmentofthetherapeuticagentsforallergies,assuchIgGscouldcompetewithallergenicIgEfortheallergen(Crooteetal.,2018).

Antibodyfirstpublishedin:Crooteetal.High-affinityallergen-specifichumanantibodiesclonedfromsingleIgEBcelltranscriptomes.Science.2018Dec14;362(6420):1306-1309.doi:10.1126/science.aau2599.PMID:30545888Noteonpublication:ThisarticledescribesthegenerationandcharacterisationofthePA12P3D08antibody.